Despite this, the venom likely contained some fragments of OvCNPs. Search in Google ScholarĤ1 When we collected platypus venom fluid from living specimens, it was immediately frozen and stored at low temperature to avoid decomposition. In Comprehensive Natural Products, 2nd ed., K. 379–387, Nova Science Publishers, New York (2007). In D-Amino Acids: A New Frontier in Amino Acid and Protein Research – Practical Methods and Protocols, P. The Natural History of Shrews, Cornell University Press, Ithaca, NY (1990). These results strongly indicated that platypus venom contains tissue kallikrein-like protease(s), and its proteolytic activity might synergistically contribute to toxicity through the specific cleavage of other venom constituents.ġ M. We also established that its proteinous venom fraction strongly hydrolyzed Pro–Phe–Arg–MCA and cleaved a human low-molecular-weight kininogen (LK), similar to porcine pancreas kallikrein. This heptapeptide induced a significant increase in i in IMR-32 cells at 75 μM had relatively specific affinities for glutamate, histamine, and GABA A receptors and facilitated neurogenic twitching in guinea pig ileum specimens at 30 μM. Guided by this assay, we identified 11 unique peptides, including peptide H–His–Asp–His–Pro–Asn–Pro–Arg–OH, which coincided with the N-terminal domain residues of Ornithorhynchus venom C-type natriuretic peptide (OvCNP). We found that crude platypus venom produced potent Ca 2+ influx in human neuroblastoma IMR-32 cells. However, the structure and function of the poison’s active compounds are still imcompletely characterized. The adult male platypus carries a spur on each hind leg, which it uses to inject competitors with poison. Among them, the duckbill platypus ( Ornithorhynchus anatinus) is one of the two venomous Australian mammals. Venomous mammals are rare, and only a few species in the orders Insectivora and Monotremata produce toxic venom.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |